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Animal Cell Technology: Basic & Applied Aspects: Proceedings by M. Yamashita (auth.), K. Nagai, M. Wachi (eds.)

By M. Yamashita (auth.), K. Nagai, M. Wachi (eds.)

Animal mobilephone know-how is a turning out to be self-discipline of mobile biology which goals not just to appreciate buildings, features and behaviors of differentiated animal cells but additionally to discover their skills for commercial and scientific reasons. The target of animal mobile know-how comprises clonal enlargement of differentiated cells with worthy skills, optimization in their tradition stipulations at the commercial scale, modulation in their skill so as successfully to provide medically and pharmaceutically vital proteins, and alertness of animal cells to gene remedy and formation of synthetic organs. This quantity supplies the readers a whole evaluate of the current state-of-the-art in Japan, a rustic the place this box is easily complicated, in addition to in Asia, Europe and the us. The court cases might be necessary for phone biologists, biochemists, molecular biologists, biochemical engineers and people in different disciplines regarding animal cellphone tradition, operating in educational environments in addition to within the biotechnology and pharmaceutical industries.

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Extra info for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the Ninth Annual Meeting of the Japanese Association for Animal Cell Technology, Yokohama, Japan, September 1–4, 1996

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Erythropoietic System Kidney Endocrine fashion Paracrine fasion Figure 5. EPO in the peripheral and the eNS 27 4. DISCUSSION In fetal stage, EPO is produced in the liver where erythropoiesis takes place. In adult, EPO produced by the kidney travels in the circulation to reach erythropietic tissues, bone marrow and spleen in the peripheral. So far the erythroid lineage has been thought to be a sole physiological target of EPO. Several lines of evidence indicate that the EPO/EPOR system independent of that in erythropoiesis i s presentin the CNS where astrocytes produce EPO and neurons express EPOR, and that EPO protected glutamate neurotoxicity (Fig.

Koury; S. , Koury; M. , Bondurant. , and Graber, S. , Quantitation of erythropoietin-producing cells in kidney of mice by in situ hybridization: correlation with hematocrit, renal erythropoietin mRNA, and serum erythropoietin concentration, Blood, 74 (1989) 645-651 6. , Tabira, T. : comparison with receptor properties of erythroid cells, J. , 268 (1993) 11208-11216 7. , Yasuda, Y. , Erythropoietin receptoris expressed in rat hippocampaland cerebral cortical neurons, and erythropoietin prevents in vitro glutamate-induced neuronal death, NeurOSCience, (1996) in press 8.

MTX), which inhibits the enzyme expressed by an encoded marker gene. Although most cells can not survive, a few that contain a higher number of selectable marker genes can grow in this medium. The copy number of the objective genes also increases with the marker gene. Thus, by increasing the drug concentration, highly productive cell lines can be obtained. However, since a suitable pattern of drug concentration increase has not been clarified, we investigated the relationship between the specific production rate and the concentration of the toxic drug MTX in the medium during the construction of recombinant CHO cells.

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